Bernhard VII of Anhalt-Zerbst (17 March 1540 – 1 March 1570), was a German prince of the House of Ascania and ruler of the principality of Anhalt-Zerbst.
He was born and died in Dessau, and was the third and youngest son of John V, Prince of Anhalt-Zerbst by his wife Margaret, daughter of Joachim I Nestor, Elector of Brandenburg.