Johan De Roo (born 5 July 1937) is a Dutch former professional road racing cyclist between 1958 and 1968.
During 11 seasons as a professional he had six victories in single-day classics, three stages of the Tour de France and one stage of the Vuelta a España.
He had 46 wins as a professional.
He was the most successful rider from Zeeland until the emergence of Jan Raas.