Phyllis Holtby (December 10, 1906 – March 21, 1993) was a Canadian musician and educator.The daughter of John Hamilton Holtby and Annie Walker, she was born in Winnipeg and studied there with Bernard Naylor and Eva Clare, in New York City with Sigismond Stojowski and Ernest Hutcheson and in Duluth with Frank Mannheimer.
Holtby also studied harpsichord in Winnipeg.
She performed with the CBC Winnipeg Orchestra, the Duluth Symphony Orchestra and the Grand Forks Symphony Orchestra.
She also gave piano and harpsichord recitals and performed on CBC Radio.
She lectured on pedagogy at the University of North Dakota and was an examiner for the University of Manitoba.