Sofie De Vuyst (born 2 April 1987) is a Belgian racing cyclist, who currently rides for UCI Women's Team Parkhotel Valkenburg.
Between 2010 and 2016 she competed in every UCI Road World Championships.
She was voted 2019 Flandrienne of the Year (best Belgian female cyclist).In September 2019, De Vuyst tested positive for an exogenous steroid in an out of competition control.
De Vuyst’s positive test came on 18 September, in the week after the Lotto Belgium Tour, where she finished seventh overall and won the mountains classification, and before Yorkshire Worlds.