Rosamma Punnoose (12 May 1913 – 28 December 2013) was an Indian independence activist, politician and lawyer.
She was the first person to be sworn in as a member of the Kerala Legislative Assembly.
She was also the first MLA in India to lose her seat following a court order, and the first person to be elected in the first-ever by-election to the assembly in 1958.
Punnoose was also the first pro tem Speaker of the Kerala Legislative Assembly.