Anna of Holstein-Gottorp (27 February 1575 – 24 April 1610) was a German noblewoman, daughter of Duke Adolf of Holstein-Gottorp and Landgravine Christine of Hesse (daughter of Landgrave Philip I of Hesse).
On 28 January 1598, she married Count Enno III of East Frisia.
They had the following children:
Anna Maria, Countess of Ostfriesland (1601–1633), married Adolf Frederick I, Duke of Mecklenburg-Schwerin (1588–1658)
Rudolf Christian, Count of Ostfriesland (1602–1628)
Ulrich II, Count of Ostfriesland (1605–1648)