He was a member of the Liberal Party.
Jose Eligio Ayala was born in Mbuyapey in the department of ParaguarĂ on 4 December 1879, son of Spanish Mariano Sisa and Paraguayan Manuela de Jesus Ayala.
He was twin brother of Emilio de Jesus Ayala and brother of Eliseo's father, Juan Pablo, Juan Bautista and Manuel Sisa.
He was the father of the son of Rosaura Abelardo Gonzalez and daughter Anastasia Candelaria Duplán.