Holger Gilbert-Jespersen (22 September 1890 – 30 July 1975) was a Danish flutist, orchestral musician and academic flute teacher.
In 1926, Carl Nielsen's Flute Concerto was written for, dedicated to, and first performed by Gilbert-Jespersen in Paris.
He was a member of the Royal Danish Orchestra from 1927 to 1956 as well as a professor at the Royal Danish Academy of Music from 1927 to 1962, where he trained generations of flutists.