Gitte Karlshøj (born 14 May 1959 in Lyderslev, Stevns, Sjælland, Denmark) is a Danish Track and Field athlete, specializing in long distance running.
She ran the 3000 metres at the 1992 Summer Olympics, finishing in a non-qualifying 6th place in the qualifying round and the Marathon at the 1996 Summer Olympics, failing to finish.
She also made the final at the 1992 IAAF World Championships.
Starting her career at a fairly late age (early twenties), she teamed up with runner Flemming Jensen as her first coach and trainer.
This partnership brought her from being a recognised talent to a developed professional top athlete.
At the later part of her career she took over responsibility of own coaching and used Flemming more as a mentor.
This partnership however, faded out at the time of her late career continuation.
She studied in the United States running for Seattle Pacific University.
She has continued running into the Masters athletics age groups.
She is the current World Record holder in the W50 division at 1500 metres, 3000 metres, 5000 metres and 10000 metres.
She also has a pending W50 world record for the mile.
Gitte Karlshøj starred as a personal trainer for DR2 runners who ran the New York Marathon in Tema Saturday in November 2006.
She is a physiotherapist by Team Denmark in Aarhus and lecturer in business/sports clubs.