Mary Littlejohn Singleton (born September 20, 1926 – died December 7, 1980) was a Florida teacher and politician, serving on the Jacksonville, Florida City Council before and after the consolidation in 1968 with Duval County.
In 1967 she was one of the first two black women elected to the Council.
She was re-elected after consolidation and served until 1972.
In 1972 Singleton was elected to the state legislature, the first woman and first black elected to that body from North Florida, serving until 1976.
She ran unsuccessfully for lieutenant governor, and was appointed to state office after that, living in Tallahassee.