John Louis II, Prince of Anhalt-Zerbst (23 June 1688, in Dornburg – 5 November 1746, in Zerbst), was a German prince of the House of Ascania and ruler of the principality of Anhalt-Dornburg.
After 1742 he became ruler over the principality of Anhalt-Zerbst .
He was the eldest son of John Louis I, Prince of Anhalt-Dornburg, by his wife Christine Eleonore of Zeutsch.