Marco Ambrosio (30 May 1973 in Brescia) is a former Italian footballer who played as a goalkeeper.
Ambrosio played for multiple teams in the Italian leagues from 1991 until his retirement in 2009 (most notably with Serie A sides Atalanta, Sampdoria and Chievo), but also had brief spells in England with Chelsea, and in Switzerland with Grasshopper.
He made two appearances for his country's under-21 side during the 1993–94 season, but never made an appearance for the full national side.